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Gram negative bacilli - enterobacteriaceae
Escherichia coli
Morphology
Shape: Rod
Arrangement: Single or pairs
Size: 1-2 μm in length, 0.25-1 μm in diameter
Motility: Motile (peritrichous flagella) or non-motile
Capsule: Some strains have capsule
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Positive
Methyl_red: Positive
Vp: Negative
Citrate: Negative (most strains)
Urease: Negative
H2s_production: Negative
Tsi: A/A, gas +, H2S -
Of_glucose: Fermentative
Lactose_fermentation: Positive (most strains)
Motility_test: Positive (most strains)
Growth Conditions
Temperature_range: 15-45°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: Pink colonies (lactose fermenter)
Blood_agar: Gray, moist colonies, some strains hemolytic
Klebsiella pneumoniae
Morphology
Shape: Rod
Arrangement: Single
Size: 1-2 μm length, 0.5-0.8 μm diameter
Motility: Non-motile
Capsule: Thick polysaccharide capsule
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Negative (K. pneumoniae), Positive in some subspecies
Methyl_red: Negative
Vp: Positive
Citrate: Positive
Urease: Positive
H2s_production: Negative
Tsi: A/A, gas +, H2S -
Of_glucose: Fermentative
Lactose_fermentation: Positive
Motility_test: Negative
Growth Conditions
Temperature_range: 10-42°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: Pink, mucoid colonies
Blood_agar: Large, mucoid colonies, non-hemolytic
Salmonella enterica
Morphology
Shape: Rod
Arrangement: Single
Size: 1-3 μm length, 0.5-0.8 μm diameter
Motility: Motile (peritrichous flagella)
Capsule: Vi antigen in some strains
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Negative
Methyl_red: Positive
Vp: Negative
Citrate: Positive
Urease: Negative
H2s_production: Positive
Tsi: K/A, gas +, H2S +
Of_glucose: Fermentative
Lactose_fermentation: Negative
Motility_test: Positive
Growth Conditions
Temperature_range: 15-43°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: Pale colonies (non-lactose fermenter)
Blood_agar: Smooth, moist colonies, usually non-hemolytic
Proteus mirabilis
Morphology
Shape: Rod
Arrangement: Single
Size: 1-3 μm length, 0.5-0.8 μm diameter
Motility: Highly motile (swarming motility)
Capsule: Absent
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Negative
Methyl_red: Positive
Vp: Negative
Citrate: Positive
Urease: Positive
H2s_production: Positive
Tsi: K/A, gas +, H2S +
Of_glucose: Fermentative
Lactose_fermentation: Negative
Motility_test: Positive
Growth Conditions
Temperature_range: 10-43°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: Pale colonies
Blood_agar: Swarming growth, strong odor
Gram positive cocci - clusters
Staphylococcus aureus
Morphology
Shape: Coccus
Arrangement: Clusters
Size: 0.5-1 μm in diameter
Motility: Non-motile
Capsule: Present in some strains
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Negative
Methyl_red: Variable
Vp: Positive
Citrate: Variable
Urease: Positive (some strains)
H2s_production: Negative
Tsi: A/A, gas -, H2S -
Of_glucose: Fermentative
Lactose_fermentation: Negative
Motility_test: Negative
Growth Conditions
Temperature_range: 15-45°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: No growth
Blood_agar: Golden-yellow, beta-hemolytic colonies
Gram negative bacilli - non-fermenter
Pseudomonas aeruginosa
Morphology
Shape: Rod
Arrangement: Single
Size: 1.5-3 μm in length, 0.5-1 μm in diameter
Motility: Motile (polar flagella)
Capsule: Slime layer in some strains
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Positive
Catalase: Positive
Indole: Negative
Methyl_red: Negative
Vp: Negative
Citrate: Positive
Urease: Negative
H2s_production: Negative
Tsi: K/K, gas -, H2S -
Of_glucose: Oxidative
Lactose_fermentation: Negative
Motility_test: Positive
Growth Conditions
Temperature_range: 25-42°C
Optimal_temp: 37°C
Oxygen: Obligate aerobe (can use nitrate anaerobically)
Macconkey: Pale colonies (non-lactose fermenter)
Blood_agar: Large, flat colonies with metallic sheen, beta-hemolytic
Gram positive cocci - chains
Enterococcus faecalis
Morphology
Shape: Coccus
Arrangement: Pairs and short chains
Size: 0.6-2.0 μm diameter
Motility: Non-motile
Capsule: Absent
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Negative (weak reaction possible)
Indole: Negative
Methyl_red: Positive
Vp: Variable
Citrate: Variable
Urease: Negative
H2s_production: Negative
Tsi: A/A, gas -, H2S -
Of_glucose: Fermentative
Lactose_fermentation: Positive
Motility_test: Negative
Growth Conditions
Temperature_range: 10-45°C
Optimal_temp: 37°C
Oxygen: Facultative anaerobe
Macconkey: Poor or no growth
Blood_agar: Small, translucent colonies, gamma or alpha hemolysis
Gram positive bacilli - non-spore forming
Listeria monocytogenes
Morphology
Shape: Rod
Arrangement: Single or short chains
Size: 0.5-2 μm length, 0.5 μm diameter
Motility: Tumbling motility at room temp
Capsule: Absent
Spores: Non-spore forming
Biochemical Characteristics
Oxidase: Negative
Catalase: Positive
Indole: Negative
Methyl_red: Variable
Vp: Positive
Citrate: Negative
Urease: Negative
H2s_production: Negative
Tsi: A/A, gas -, H2S -
Of_glucose: Fermentative
Lactose_fermentation: Negative
Motility_test: Positive at 25°C
Growth Conditions
Temperature_range: 0-45°C
Optimal_temp: 30-37°C
Oxygen: Facultative anaerobe
Macconkey: No growth
Blood_agar: Small colonies, narrow beta hemolysis
Bacteria - gram positive cocci
Streptococcus pyogenes
Biochemical Characteristics
Catalase: Negative
Hemolysis: Beta
Bacitracin_sensitivity: Sensitive
Pyr: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Causes strep throat, scarlet fever, rheumatic fever
Streptococcus pneumoniae
Biochemical Characteristics
Catalase: Negative
Optochin_sensitivity: Sensitive
Bile_solubility: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Capsule: Polysaccharide capsule
Pathogenicity: Causes pneumonia, meningitis, otitis media
Streptococcus agalactiae
Biochemical Characteristics
Catalase: Negative
Hemolysis: Beta
Camp_test: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Neonatal sepsis, meningitis
Streptococcus mutans
Biochemical Characteristics
Catalase: Negative
Lactose_fermentation: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Dental caries
Bacteria - gram negative cocci
Neisseria gonorrhoeae
Biochemical Characteristics
Oxidase: Positive
Glucose_utilization: Positive
Maltose_utilization: Negative
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Gonorrhea, neonatal conjunctivitis
Neisseria meningitidis
Biochemical Characteristics
Oxidase: Positive
Glucose_utilization: Positive
Maltose_utilization: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Capsule: Polysaccharide capsule
Pathogenicity: Meningitis, septicemia
Bacteria - gram negative rods
Shigella dysenteriae
Biochemical Characteristics
Oxidase: Negative
Lactose_fermentation: Negative
H2s_production: Negative
Urease: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Bacillary dysentery
Salmonella Typhi
Biochemical Characteristics
Oxidase: Negative
Lactose_fermentation: Negative
H2s_production: Positive
Urease: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Typhoid fever
Legionella pneumophila
Biochemical Characteristics
Oxidase: Positive
Growth_requirement: Cysteine and iron
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Legionnaires' disease
Yersinia pestis
Biochemical Characteristics
Oxidase: Negative
Urease: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Plague
Enterobacter cloacae
Biochemical Characteristics
Oxidase: Negative
Lactose_fermentation: Positive
Urease: Variable
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Hospital-acquired infections, UTIs
Escherichia coli O157:H7
Biochemical Characteristics
Oxidase: Negative
Lactose_fermentation: Positive
Sorbitol_fermentation: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Hemorrhagic colitis, hemolytic uremic syndrome
Shigella sonnei
Biochemical Characteristics
Oxidase: Negative
Lactose_fermentation: Negative
Urease: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Shigellosis
Bacteria - gram negative curved rods
Vibrio cholerae
Biochemical Characteristics
Oxidase: Positive
Glucose_fermentation: Positive
Lactose_fermentation: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Motility: Single polar flagellum
Pathogenicity: Cholera
Campylobacter jejuni
Biochemical Characteristics
Oxidase: Positive
Catalase: Positive
Hippurate_hydrolysis: Positive
Other Characteristics
Oxygen_requirement: Microaerophilic
Pathogenicity: Gastroenteritis, Guillain-Barré syndrome
Vibrio parahaemolyticus
Biochemical Characteristics
Oxidase: Positive
Halophilic: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Gastroenteritis from seafood
Bacteria - gram negative coccobacilli
Haemophilus influenzae
Biochemical Characteristics
Oxidase: Positive
X_and_v_factor_requirement: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Capsule: Polysaccharide capsule in type b
Pathogenicity: Meningitis, epiglottitis
Acinetobacter baumannii
Biochemical Characteristics
Oxidase: Negative
Glucose_oxidation: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Nosocomial infections, ventilator-associated pneumonia
Brucella melitensis
Biochemical Characteristics
Oxidase: Positive
Urease: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Brucellosis
Bacteria - spirochetes
Treponema pallidum
Biochemical Characteristics
Motility: Corkscrew motility
Other Characteristics
Oxygen_requirement: Microaerophilic
Pathogenicity: Syphilis
Borrelia burgdorferi
Biochemical Characteristics
Motility: Corkscrew motility
Other Characteristics
Oxygen_requirement: Microaerophilic
Pathogenicity: Lyme disease
Leptospira interrogans
Biochemical Characteristics
Motility: Corkscrew motility
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Leptospirosis
Fungi - yeast
Candida albicans
Biochemical Characteristics
Germ_tube_test: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Oral thrush, vaginal candidiasis
Cryptococcus neoformans
Biochemical Characteristics
Urease: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Meningitis in immunocompromised hosts
Candida glabrata
Biochemical Characteristics
Germ_tube_test: Negative
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Urinary tract infections, bloodstream infections
Fungi - mold
Aspergillus fumigatus
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Aspergillosis, allergic bronchopulmonary aspergillosis
Fungi - dimorphic
Histoplasma capsulatum
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Histoplasmosis
Bacteria
Bacillus cereus
Mycoplasma pneumoniae
Rickettsia rickettsii
Francisella tularensis
Bordetella pertussis
Helicobacter pylori
Clostridium difficile
Bacteria - acid-fast bacilli
Mycobacterium tuberculosis
Biochemical Characteristics
Niacin_test: Positive
Nitrate_reduction: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Tuberculosis
Bacteria - gram positive bacilli
Bacillus cereus
Biochemical Characteristics
Catalase: Positive
Spore_formation: Positive
Lecithinase: Positive
Other Characteristics
Oxygen_requirement: Facultative anaerobe
Pathogenicity: Food poisoning, endophthalmitis
Corynebacterium diphtheriae
Biochemical Characteristics
Catalase: Positive
Urease: Negative
Toxin_production: Positive (diphtheria toxin)
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Diphtheria
Clostridium perfringens
Biochemical Characteristics
Catalase: Negative
Spore_formation: Positive
Lecithinase: Positive
Other Characteristics
Oxygen_requirement: Obligate anaerobe
Pathogenicity: Gas gangrene, food poisoning
Propionibacterium acnes
Biochemical Characteristics
Catalase: Positive
Indole: Variable
Other Characteristics
Oxygen_requirement: Anaerobe
Pathogenicity: Acne, opportunistic infections
Bacteria - intracellular
Chlamydia trachomatis
Biochemical Characteristics
Giemsa_stain: Positive
Other Characteristics
Oxygen_requirement: Obligate intracellular
Pathogenicity: Chlamydia, trachoma, lymphogranuloma venereum
Bacteria - no cell wall
Mycoplasma pneumoniae
Biochemical Characteristics
Glucose_fermentation: Positive
Other Characteristics
Oxygen_requirement: Aerobe
Pathogenicity: Atypical pneumonia